POLAROGRAPHIC DETERMINATION OF DEHYDROISOANDROSTERONE AND OTHER 3-HYDROXY-Δ5-STEROIDS

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Determination of dehydroisoandrosterone and 17-ketosteroids.

Methods for the quantitative determination of steroids have been developed, based on their specific chemical or physicochemical properties. The Zimmermann reaction (1) and the Kober reaction (2) have been found useful in the quantitative determination of certain individual steroids occurring in urine. Modifications of these reactions have been reported by Callow et al. (3), Holtorff and Koch (4...

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Determination of Dehydroisoandrosterone and 17 - Ketosteroids

Methods for the quantitative determination of steroids have been developed, based on their specific chemical or physicochemical properties. The Zimmermann reaction (1) and the Kober reaction (2) have been found useful in the quantitative determination of certain individual steroids occurring in urine. Modifications of these reactions have been reported by Callow et al. (3), Holtorff and Koch (4...

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The conversion of dehydroisoandrosterone to its ,B-D-glucopyranoside is readily effected by surviving slices of the potato tuber at pH 8. This reaction is largely limited to dehydroisoandrosterone and isoandrosterone, and is slowly reversed at pH 4 as the result of weak P-glucosidase-like activity. Potatoes also contain an active /?-glucuronidase which differs from known preparations in possess...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1941

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)72907-4